Sarabjeet kour Sudan

8 Questions 33 Answers 0 Followers

Questions related from Sarabjeet kour Sudan

Can anyone suggest me that why protein is not active after purification?

I am purifying a protein which is showing activity in sonicated supernatant but when i am purifying it using ion exchange, eluted fraction is losing the activity.I did dialysis also even then the...

12 December 2016 2,566 5 View

How to visualize the bands of protein on SDS gel other than by coomassie staning?

I am doing purification of enzyme from culturables.Problem is that after GFC when i am running the protein on gel,band is not visible but the protein is there as when i am performing assay and...

08 August 2016 8,610 6 View

What can be the reason of not getting induction in expression hosts ??

I am trying to clone a gene and go for protein expression. But it is not getting induced . Although I checked my insert by digestion and then by sequencing before doing transformation in...

02 February 2016 7,364 6 View

How to preserve thermophilic organisms?

I had preserved my thermophilic organisms by preparing glycerol stocks but everytime when i am reviving it they are not growing again on plates ...please suggest some methods

09 September 2014 2,643 6 View

Why is the DNSA method showing no activity of my isolate although it shows activity on a starch azure plate?

I have an isolate that shows amylase activity on a starch azure plate incubated at 70°C but when I grew this isolate in broth, centrifuged it, and took its supernatant for performing assays, there...

02 February 2014 439 5 View

Heavy metals detoxifying enzymes assay.

I want to check the activity of enzymes responsible for heavy metals detoxification can anyone tell me the assay which i should use for the same.

04 April 2013 2,557 11 View

Is it possible to use a fosmid vector for an insert size of 10KB?

I am using fosmid vector for 40kb insert, but can I use same for insert of less size?

03 March 2013 3,863 2 View

How to separate ligated fosmid vector from genomic DNA of host cells

I am trying to isolate Fosmid DNA from my clones by using a Fosmid purification kit but every time I am getting two bands on the gel so I am not able to give my samples for sequencing. How can I...

12 December 2012 308 23 View