I need to transport embryonic organs fixed in 4% PFA and previously stained in b-gal solution (LacZ) to another country. Our objective is to embeed the tissues in paraffin. Is 4% PFA the best solution for transport of these organs in leak/spill-proof containers? Any experience/insights would be very helpful.
Once the tissue is thoroughly fixed, it should be fine for transport in PBS for example. This would save you from making and transporting copious amounts of toxic PFA! I would do a trial run with a small piece of tissue first to make sure before committing to large shipments.
Hello,
first of all: How should the material be shipped?
In your own car, by Train, by postal mail, airplane etc.....
If you Transport them in your own car or by Train in your luggage I think that 4% PFA is not a Problem if the tubes are proof.
and second from which Country to which Country? There you have to consider the official regulations of the different countries.
For Transportation of perfused rat brains by postal mail from Germany to Bulgaria (2-3 days of shipment, both EU-countries) we reduced the PFA concentration to 1%. The final immunohistochemical results were good.
Hope this helps
Kind regards
Stefan
Transporting of specimens in formalin is an usual procedure. You have to check the regulations of the transporting company (postal office?) for the specific country.
One container for the specimen within a second container tightly closed. And the containers put in a package, that prevent the containers from braking. In Austria and Germany specimens in formalin for diagnostic purposes need no further labelling of the package. It is an exception and not classified as biohazard.
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