Dear all,

I have a slightly odd question regarding immunogold silver enhancement. I am aiming to look at 2 targets in the same tissue section, but I need a way to differentiate them using metal tagged antibodies. I have unlabelled primaries of different host species and 2 secondary antibodies that are tagged with gold nanoparticles.

Is it possible to silver enhance one secondary antibody, wash away the enhancement reagents and then proceed with the next secondary incubation? In this way I would hope to have one target labelled with gold/silver and another target labelled with only gold.

I am only a Masters student so budget is limited - purchasing any more reagents for alternative approaches such as MaxPar kits or silver tagged antibodies isn't really an option.

Please let me know!

Kind regards,

Charlie

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