For the reconstruction of past climate two things are most important: one is the reproducibility of data, and second is authenticity of age model, or in other ways we can say age model is the backbone for the reconstruction of past climate.

In carbonate terrain, its very hard to establish the good age model, because lake catchments containing geologically old carbonate (e.g. limestone) can be a source of infinitely old, 14C-depleted, carbon for lake or pond water. So in that area, bacteria are the good source of study because once we trace there evolutionary trend we are able to correlate them with past climate.

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