How to resuspend cultured adult rat myocytes without damaging the cells?

Hello everyone, Which is the best way to resuspend a culture of adult rat ventricular cardiomyocytes without damaging the cells integrity? This is what I am trying to do. I isolate ventricular...

31 December 2019 1,080 2 View

How can I analyse fluorescence images of double stained cells?

I have a set of cells double stained in this manner: first the cells has been live-stained to visualize the protein of interest at the membrane (named: SURFACE). Then, cells have been fixed and...

06 July 2018 7,032 7 View

Why is the gain continuously changing during recording in Clampex?

Hello everyone, I've set up Clampex 10.5 for an Axopatch 200B connected to a 1550A Digidata and I have this problem: the gain on Clampex is changing randomly. This happens both while recording...

01 February 2018 7,697 4 View

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04 May 2017 6,171 5 View

Does anyone know why hiPS-CMs tend to die upon contact?

I'm doing some perforated patch-clamp on human-derived IPS cardiomyocytes. Most of the cells die (by exploding/melting in vesicles) as soon as I touch them with the tip of the glass pipette. Does...

09 October 2016 2,990 5 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

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02 March 2021 3,060 3 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View