Dario Melgari

6 Questions 2 Answers 0 Followers

Questions related from Dario Melgari

How to resuspend cultured adult rat myocytes without damaging the cells?

Hello everyone, Which is the best way to resuspend a culture of adult rat ventricular cardiomyocytes without damaging the cells integrity? This is what I am trying to do. I isolate ventricular...

01 January 2020 1,188 2 View

How can I analyse fluorescence images of double stained cells?

I have a set of cells double stained in this manner: first the cells has been live-stained to visualize the protein of interest at the membrane (named: SURFACE). Then, cells have been fixed and...

07 July 2018 7,131 7 View

Why is the gain continuously changing during recording in Clampex?

Hello everyone, I've set up Clampex 10.5 for an Axopatch 200B connected to a 1550A Digidata and I have this problem: the gain on Clampex is changing randomly. This happens both while recording...

02 February 2018 7,825 4 View

Is it possible to purify clathrin-coated vesicles from atrial cardiomyocytes? If so, can anyone suggest a good protocol?

I'm thinking about to possibility of purify CCVs from atrial CMs (rat). The available protocols in the literature are focused on cell lines or on tissues from organs such as brain and liver. Does...

01 January 2018 2,645 0 View

ECOS DH5α growth problem.

I'm doing ligation (ligation high v.2) + transformation (ECOS DH5α) of quite a big plasmid (12.000bp, the fragment with the mutation is roughly 3000bp). I'm transforming 2 uL of ligation product...

05 May 2017 6,262 5 View

Does anyone know why hiPS-CMs tend to die upon contact?

I'm doing some perforated patch-clamp on human-derived IPS cardiomyocytes. Most of the cells die (by exploding/melting in vesicles) as soon as I touch them with the tip of the glass pipette. Does...

10 October 2016 3,083 5 View