11 June 2019 8 545 Report

What result if can

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My question concerns MTT cell viability assay?

Despite not having cells in the media, I am getting purple color. I have tried many troubleshooting methods, varying media types, and even different MTTs from different companies to figure out the...

21 July 2024 9,914 1 View

Guidance needed for preparing the hydrogel samples for the XRD instrument?

I'm working with a hydrogel sample and I'd like to perform XRD analysis. Can anyone offer guidance on preparing the hydrogel for the XRD instrument? Specifically, I'm unsure about the best method...

20 July 2024 3,611 4 View

How can I extract the mathematical equation from existing Neural Network Model?

There exists a neural network model designed to predict a specific output, detailed in a published article. The model comprises 14 inputs, each normalized with minimum and maximum parameters...

14 July 2024 2,714 3 View

Why does my IVT gRNA product gives HMW smear in gel?

We are using MEGAshortscript™ T7 Transcription Kit for transcribing gRNAs from linearized plasmid templates. (MSP3485 digested with HindIII) (https://www.addgene.org/140082/) We let the reaction...

01 July 2024 6,342 0 View

How can Abaqus CAE be utilized to assess the variation in wall thickness throughout a plastic bottle following a blow molding simulation?

Hello ResearchGate Community, How can Abaqus CAE be utilized to assess the variation in wall thickness throughout a plastic bottle following a blow molding simulation?

26 June 2024 9,281 4 View

JCR 2024 Impact Factor Download File-Factors caused decline in IF are?

Why the IF of almost all journals declined so much? For the file download go to https://www.researchgate.net/post/JCR_Impact_Factor_2024-Factors_causes_decline_in_IF

21 June 2024 3,358 0 View

When use a HOMER Pro to design hybrid system PV/electrolyzer/hydrogen tank. How can I use all electrical load to power the electrolyzer?

Simulating a Hybrid System with PV, Grid, Electrolyzer (No Load) in HOMER. here is a question about how to use HOMER Pro to design a solar PV system for water electrolysis using electrolyzer for...

05 June 2024 5,609 0 View

Can I dilute the serum sample further than stated in the manual as long as the analyte concentration remains within the KIT linear range?

I want to measure a protein in serum using an ELISA kit. This kit allows serum samples to be diluted 200 to 3200 times, but I want to use this kit to titrate higher concentrations of protein. Can...

03 June 2024 4,937 0 View

Expert questionnaire, Is it possible for people with a specialty in music psychology to evaluate this questionnaire? Through the link below?

It is possible for people specialized in music psychology to evaluate this questionnaire Through the link below Thank you https://forms.gle/qzoD7jN2UDUhMjwQ7

02 June 2024 9,546 0 View

How can we extract DNA from yeast isolates without using any lysis buffer or lysis enzymes? Is there any manual protocol?

?K

31 May 2024 548 4 View

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