22 November 2017 1 8K Report

I have a plasmid which was restricted with two restriction enzymes to remove the insert. Now, I am attempting to generate an empty vector from the original plasmid. I plan to ligate the two ends using short oligos (~20bp) which would have restriction sites for different restriction enzymes (similar to multiple cloning site). I am unsure if this approach works. If it does, has anyone tried this idea before or is there any other way I can ligate my plasmid.

P.S. I checked the list of compatible restriction enzymes and unfortunately I cannot use any of them. Also, I have a tag just before my 5' restriction site which i cannot lose.

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