As we want to look for inhaled materials in the lungs, we don't want to use instilled fixatives, which could move the material to other compartments. Is it possible to keep the lung structure in this way? Or do you have any other suggestions?
Thanks, Monika. Do you know if Cryomatrix is any different from e.g. formalin for this type of preservation? I reformulated the question to reflect the potential problem of not wanting to instil any fixative into the lungs. BR, Jitka
Hello Jitka,
I have had to freeze samples making sure the structure was preserved and there was no addition of a fixative agent. The Cryomatrix that Monika is referring to is most definitely not a fixative, so that's good. But, that being said, it is a bit dense, and if you fill the lungs with air before, you might have some deflation due to the heaviness of the Cryomatrix. If you want to preserve the structure of the lung, you can instill the Cryomatrix prior to freezing and that way the structure will be preserved.
good luck!
toby
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