Hello, I am currently growing a 3T3L1 cell.
When I first thawed the frozen cell, the live cell percent was 99%. (I counted the cell number via cell counting machine) However, when I did the subculture for the first time, the live cell percent was about 65%. When I did the subculture, the cell confluence was a bit high for 3T3L1 cell I think.. Also I think it's the reason of the low live cell percent. I am currently trying to heighten the live cell percent by performing subculture when the confluence isn't so high but it isn't quite working.. (For Hepa cell it worked)
Is it impossible to heighten the live cell percent of a 3T3L1 cell once the live cell percent is lowered?