Hyun Jeong Yoon

6 Questions 3 Answers 0 Followers

Questions related from Hyun Jeong Yoon

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

12 August 2024 6,958 3 View

What is wrong with the mitochondria isolation protocol?

Hello I am currently trying to set up a new protocol. Our lab is planning to perform study about mitochondria, and we still couldn't set up a proper protocol. The kit we used is from abcam...

06 November 2023 9,652 0 View

Is there any mitochondrial DNA isolation kit for serum?

Hello I am currently searching method for isolating mitochondrial DNA from serum. I've tried to find a commercial kit but I think mitochondrial DNA kits are for tissue or cell samples.. Does...

27 February 2023 6,150 0 View

Is the 3T3-L1 differentiation successful??

Hello I am currently differentiating a 3T3-L1 cell. The passage number is 17, and I've already treated the cell with differentiation media(insulin+dexamethasone+IBMX) for two days(day 0) and...

30 August 2022 5,181 5 View

Is it possible to increase the live cell percent of 3T3L1 cell?

Hello, I am currently growing a 3T3L1 cell. When I first thawed the frozen cell, the live cell percent was 99%. (I counted the cell number via cell counting machine) However, when I did the...

27 April 2022 3,530 0 View

Why can't I see half of the ladder in my gel after transferring to membrane?

Hello I did an electrophoresis for the first time, and transferred the proteins to PVDF membrane. I saw the entire ladder in the gel before transferring. However, after the transfer, half of the...

22 February 2022 3,259 4 View