I used LDH cytotoxicity kit from roche to evaluate LDH leakage
With the remaining lysed cells used for Maximal LDH, I want to extract RNA ( Trizol or RLT) is there any conter argument?
RNA is highly labile, and will quickly degrade if you do not protect it. This will lead to false negative results. If you are referring to an experiment already conducted I would say No. If you are referring to an upcommng experiment you need to chemically stabilize the RNA as this will (hopefully) maintain the gene expression profile.
I traditionally, snap freeze cells in liquid nitrogen before freezing at -80, followed by a kit RNA extraction.
Thanks Nicklas for your anwer. As the LDH measurement is already performed I will try classical extraction followed by QC with bioanalyser nano chips the RIN number will be a decisive information
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