Hi,
I am trying to grow Lactobacillus acidophilus cells in MRS broth, and I use Nitrogen to maintain anaerobic conditions. Looking at the cells, I suspect a Bifidobacterium contamination. Is this possible? Does Bifidobacterium grow on MRS? How can I prevent it?
Thanks for your insights!
Thje Bf.animalis ssp.lactis easily can grow in MRS under similar conditions as acidophilus and it may well also have been a contaminatino from the supplier of the Bif.!
MRS media is also used for counting of the Bf.a.ssp.lactis!
Yes, The Bifidobacterium SPP. can grow in MRS and increased the growth of bacteria under anaerobic condition .
You can use MRS-salicin or MRS-mannitol.
Thank you all for the answers.
Follow up question :
The contamination I see is Y shaped cells among rod shaped cells (acidophilus). So I assumed its Bifido. But B.animalis are not Y shaped right?
Thanks again for your inputs!
L.acidophilus can sometimes look nearly as Bif. and the BLC can look like a Lb., so not so easy to check with microscope!
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