As any other aerotollerant organism, I guess, it can grow in CO2 incubator, maybe slowlier then it would do in anaerobic conditions. I think that you should keep a temperature between 30°C and 37°C. For the O2 conditions I would try between 3% and 8%, no more then 10% anyway... But I actually never done it so these are only suppositions. If you try let me know.

Has anyone else ever done it?

Greetings.

P.S. if you are looking for some cheap trick, you could try to grow it with the "box&candle trick", put the culture into a box that can be hermetically closed, with a burinig candle, close carefully and wait for the candle to off. That should be a good anaerobic condition!

Thanks Ramona for your valuable suggession.......I will try and will inform you about its results.

Ok, I'm really curious!

inoculate Pseudomonas aeruginosa in a different agar plate and put it near of P. acne inoculated plate. P. aeruginosa spends O2 If you are using CO2 incubator you dont need extra intervention. Wait for 7-10 days for first culture. Then its subcultures can easy grow even within 4-5 days. BHI best medium. good luck

I'm really interested to hear the results too!  In one of my classes, we've used P. acnes as our model anaerobe, and could only get it to grow using anaerobic pouches (Oxoid's system).   I use trypic soy agar + 5% sheep blood, if that matters.  It wouldn't grow, or at least not well, using the campy microaerophilic pouches, which should produce results as good as or better than a candle jar.  Speaking of candle jars, I'm linking a recent cool find that I'm wanting to try out, and that might be helpful for your situation too.

http://www.ijmm.org/article.asp?issn=0255-0857;year=2013;volume=31;issue=2;spage=173;epage=176;aulast=Maiti

Could you tell me which one of  Oxoid anaerobic system, did you use for P acnes? Thank you!