Is it possible to find protein interfaces in MOE?

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Which Indian sectorial Indices data is better BSE or NSE?

I am interested in studying the volatility and seasonality in the Indian stock markets across sectors. Which stock exchange provides more robust coverage of sectorial indices Bombay Stock Exchange...

11 January 2024 482 3 View

How different Isoelectric point of glycosylated protein monomer vs multimer?

Currently, I use icIEF technique to determine pI of a protein. I see several peaks (more than 15) in profile. I want to use the neat protein to determine its pI and do not want to introduce any...

05 October 2023 8,649 5 View

Which is better- gene knock-out or gene knock-down ?

How do I choose while designing an expert?

01 October 2023 166 4 View

What is difference in LORAWAN class A , B , C devices and what is spreading factor relation with time to air??

09 September 2023 8,533 2 View

What are swerling target 0 , 1, 2,3,4 In Radars?

What is swerling target 0 , 1, 2,3,4 In Radars?

22 April 2023 9,971 6 View

What options do I have if the assumption of the linearity of the logit is not met for one predictor in a binary logistic regression model?

I’ve created a binary logistic regression model with two continuous independent variables (age and annual salary) to predict car purchase (0-not purchased and 1 for puchased) using SPSS. Testing...

07 March 2023 1,189 3 View

When we Launch rocket to put our satellite in orbit , how it is put in orbit so accurately?

When we Launch rocket to put our satellite in orbit , how it is put in orbit so accurately? Because as I know their are debries in Leo, Meo & Geo so how they find their way out by going through...

24 February 2023 1,592 6 View

How do our Radars get the location of the Planes , jets or Missiles when they are moving ?

How do our Radars get the location of the Planes , jets or Missiles when they are moving ? like how does the stationory radar works?

22 February 2023 4,710 3 View

how far can i take gravity assist manoeuvre and how it works?

21 February 2023 6,514 3 View

How does stealth technology works?

How does stealth technology works in Fighter jets? and my radar is not able to track the planes in this ? also how does chaff works? to avoid radar detection?

20 February 2023 7,259 6 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Determining the worth of a point improvement in Hamilton Depression Scale?

Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...

05 August 2024 3,246 1 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?

29 July 2024 4,570 0 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

My cfDNA extraction protocol using silica beads works with plasma collected in COD SAGM bags but not with plasma collected in K2 EDTA tubes. Why so?

I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...

28 July 2024 3,559 0 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View