Are there any restrictions and complications in analysis of such a material?
You can do it. There are many published works on this topic. We are working with COI, ITS2 and 28S and we have no problems with dry insects (age < 10 year) in some cases we also amplified DNA from insects older than (80 years). However, in old insects DNA is often fragmented so it is more likely a failure. Usually from dried insects if we are working with COI we amplify more short fragments to overcome this problem.
You can do it. There are many published works on this topic. We are working with COI, ITS2 and 28S and we have no problems with dry insects (age < 10 year) in some cases we also amplified DNA from insects older than (80 years). However, in old insects DNA is often fragmented so it is more likely a failure. Usually from dried insects if we are working with COI we amplify more short fragments to overcome this problem.
Yes it is possible.. You can try the the freezing method which has been applied on dried and fresh, tiny and economically important insect samples, i.e. On adults and larvae of wasps, fruit flies and thrips. You can see the attachment..
Hi,
I agree with Umberto; there should be not a problem to recover DNA from dry insect specimens collected in the same season. You can easily find many papers on this topic in the internet; for instance, we have recovered mt DNA even from the dried museum specimen of true-bug collected in 1932 (see the attachment),
Best regards,
Jerzy
It also depends how fast the drying process was (the faster the better). Therefore with small insects with a thin and not very sclerosed integument you may get better preserved DNA compared to large insects with a strong integument.
To avoid too much damaged DNA you may use only parts of the insect that dry fast (e.g. legs, antennae), if the content is sufficient.
DNA studies could be done from the dry preserved insects even after decades.
A landmark study has carried out that through the DNA amplification resistance to insecticides can be detected in pinned specimens of Australian sheep blowflies that were collected before the introduction of the insecticide.
plz see: C.J. Hartley et al.(Amplification of DNA from preserved specimens shows blowflies were preadapted for the rapid evolution of insecticide resistance, Proc. Natl. Acad. Sci. U. S. A., 103 (2006), pp. 8757–8762)
Methods for the preservation of insects for DNA studies
RJ Post, PK Flook, AL Millest - Biochemical Systematics and Ecology, 1993 -
DNA amplification from pin-mounted bumble bees (Bombus) in a museum collection: effects of fragment size and specimen age on successful PCR
JP Strange, J Knoblett, T Griswold - Apidologie, 2009
I have good results with Ticks using a Kit:
http://www.analytik-jena.de/en/life-science/products/prod/cat/isolation-of-microbial-dna/prod/blackprep-tick-dna-rna-kit.html
RNA you can get only from fresh tissue, but DNA, inc. mtDNA is good for PCR etc.
If home-made methods do not work adequately, you can try with commercial kits (qiagen dneasy tissue and blood kit) to extract good quality DNA.
Yes it is possible. There is a another paper on it with the bar coding;
Ashfaq, et al. (2014) Analyzing Mosquito (Diptera: Culicidae) Diversity in Pakistan by DNA Barcoding. PLoSONE 9(5): e97268. doi:10.1371.
To believe on the Day of Resurrection, present day scientific discoveries invite us to such phenomenon. Please read my related article on the Research Gate, entitled "CODE OF HUMAN LI F E BEFORE BI R TH,DURING LI F E AND AF TE R DEATH" focus on human DNA in the light of Science and Quran'.
It is possible, as long as they are kept dry to avoid fungus, if you keep the specimens at room temperature avoid storage with napthalene balls, instead use silica gel spheres. Depending on the size of insect you will need a different amount of tissue, people often use 1-3 legs (butterflies, mosquitoes, beetles, bees)
First let me clear that I am not an entomologist but when I Googled I found following links which may be useful to you.
1)Tropical insect collections and DNA extraction, using ...
www.mapress.com/zootaxa/2013/f/z03694p399f.pdf
by C DALE - 2013 - Related articles
2How useful is DNA extracted from the legs of archived insects for microsatellite-based population genetic analyses?
Phillip C. Watts, David J. Thompson, Katherine A. Allen, Stephen J. Kemp
Journal of Insect Conservation (Impact Factor: 1.8). 05/2007; 11(2):195-198. DOI:10.1007/s10841-006-9024-y
3)Download free PDF - Journal of Insect Science
www.insectscience.org/14.62/i1536-2442-14-62.pdf
by B Tiroesele1a
May 2, 2014 - Population structure, genetic variability, and gene flow of the .... extensive descriptions and analyses of insects ... DNA extraction by removing the gut, abdo- ... and the pellet was then air dried at room tem- .... times, with these same 24 individuals, and the ..... of the population is capable of having season-.
4)prepGEM™ DNA Extraction – Insect | TATAA Biocenter
www.tataa.com/products-page/dna.../prepgem-dna-extraction-insect/
The prepGEM™ Insect kit provides quality DNA ... For dried insects, it is advisable to break up the exoskeleton with forceps and to pre-soak the tissue overnight ...
5)FORENSIC ENTOMOLOGY : THE USE OF INSECTS
www.sfu.ca/~ganderso/forensicentomology.htm
In general, the first method is used when the corpse has been dead for between ... They usually arrive within 24 h of death if the season is suitable i.e. spring, summer .... They are cannibals so should not be placed in the same vial! .... DNA evidence is now being developed to speed up identification of immature specimens.
6)Rapid, one-step DNA extraction for insect pest identification ...
www.ncbi.nlm.nih.gov/pubmed/18459420
by SL Ball - 2008 - Cited by 15 - Related articles
Rapid, one-step DNA extraction for insect pest identification by using DNA ... taxa, whichincluded freshly collected, ethanol-preserved, and dried specimens of ...
Yes it is possible. i did it. i extracted DNA from mosquito ( Aedes aegypti) using Qiagen kit., It also depends how fast the drying process was (the faster the better).
Morphological and Molecular Identification of Dengue Fever Vector Aedes aegypti (Diptera: Culicidae) in Jeddah Governorate-Saudi Arabia.
You can also obtain sequences from older specimens if you have some knowledge of the sequence. If you for example want sequence data from multiple individuals of the same species, you can construct primer pairs based on a sequence of a fresh specimen. If you construct a number of primer pairs amplifying short overlapping fragments (100-200bp), you should be able to amplify DNA from most specimens.
As everybody else said, is possible and simple. I kept my dry insects at -80 degrees and when I want to extract DNA just use an Invitrogen reagent Triazol and I get DNA and RNA too. Quite a good quality in both cases, but as mentioned before, is plenty of publications and methods too. It is possible and easy to do. Good luck
Yes you can do itwith liquid nitrogen you can but in the liquid small bottel with forceps to hold the fly and fastly. Insert the fly and remove it it usuful for tough fly like tsetse andsuitable for molecular extractions.
I have accomplished this even on pinned large body insects like the Phyllophaga that were 5-8 years old. I agree with others above that the fresher the better. Nicely dried pinned specimens allow excision of dried flight muscles that are particularly rich in mtDNA. I used DNEasy kits from Qiagen with great success.
Freeze dried insects can be used for DNA extraction, If you collect sample from field keep them in a desiccator for slow death and then preserved them in 2% glycerol and ethanol.
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