GPT and GOT are enzymes present inside liver cells, the reason you can detect them in the serum is that liver cells are injured, membrane is broken and the enzymes are released from inside the cells to the circulation. So if you break any liver cells including healthy liver cells to obtain liver homogenate you will have high values.

 Hello Hussein H Aly sir,

when we prepare liver homogenate,  i think cells may or may not be raptured, what do you think about this

To echo what Hussein said, dissociation of liver tissue generally causes massive cell injury.  I think you will find it hard to get good values when measuring damage markers in a homogenate.  What markers are you currently measuring?

I agree with collegues. Measures of aminotransferase levels in homogenate is not marker o fpathologic  liver injury.

Adam Farmer sir, currently i am using SGOT, SGPT, ALP estimation in serum

Sorry to answer to your question with another question, but I need to know the scope of your reaserch. The evaluation of liver function tests in the serum means to assess albumin levels, coagulation factor production, pseudocholinestherase levels etc. Liver function test evaluations are used to estimate if there is a reduction of hepatocyte mass so why to perform these tests on liver tissue?

I do agree with Dr. Aly and Barone observations.

Traditional serological markers of liver function give little indication of the various underlying pathological processes but  many times you can do  good correlations with findings of liver biopsies.