Should be possible, if the peptide is the fluorescent moiety. We have used sfGFP-peptide fusions to measure the affinity of Armadillo repeat proteins of different length by fluorescence polymersiation
http://www.bioc.uzh.ch/plueckthun/pdf/APpub0391.pdf
Dear Deep,
The answer is yes.The paper that provided as a link by Annemarie. It is quite interested and useful.
Structure and Energetic Contributions of a Designed Modular Peptide-Binding Protein with Picomolar Affinity
Simon Hansen, Dirk Tremmel, Chaithanya Madhurantakam,† Christian Reichen,‡ Peer R. E. Mittl,* and Andreas Plückthun*
J. Am. Chem. Soc. 2016, 138, 3526−3532
Dear Deep,
Find in attachment, Technical Resource Guide FOURTH EDITION
Fluorescence Polarization
Find in attachment, THEMATIC REVIEW Fluorescence Polarization: Analysis
of Carbohydrate±Protein Interaction
Kazuaki Kakehi,1 Yasuo Oda, and Mitsuhiro Kinoshita
Faculty of Pharmaceutical Sciences, Kinki University,
Kowakae 3-4-1, Higashi-Osaka 577, Japan
Analytical Biochemistry 297, 111±116 (2001)
doi:10.1006/abio.2001.5309, available online at http://www.idealibrary.com on
Find in attachment, a paper on: Peptids that mimic glycosaminoglycans:
high-a¤nity ligands for a hyaluronan binding domain
Michael R. Ziebell a;b; 1, Zhan-Gong Zhao b, Bai Luo c, Yi Luo b, Eva A. Turley d,
Glenn D. Prestwich
Chemistry & Biology 8 (2001) 1081^1094
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