Should be possible, if the peptide is the fluorescent moiety. We have used sfGFP-peptide fusions to measure the affinity of Armadillo repeat proteins of different length by fluorescence polymersiation

http://www.bioc.uzh.ch/plueckthun/pdf/APpub0391.pdf

Dear Deep,

The answer is yes.The paper that  provided as a link by Annemarie. It is quite interested and useful.

Structure and Energetic Contributions of a Designed Modular Peptide-Binding Protein with Picomolar Affinity

Simon Hansen, Dirk Tremmel, Chaithanya Madhurantakam,† Christian Reichen,‡ Peer R. E. Mittl,* and Andreas Plückthun*

J. Am. Chem. Soc. 2016, 138, 3526−3532

Dear Deep,

Find in attachment, Technical Resource Guide FOURTH EDITION

Fluorescence Polarization

Find in attachment, THEMATIC REVIEW Fluorescence Polarization: Analysis

of Carbohydrate±Protein Interaction

Kazuaki Kakehi,1 Yasuo Oda, and Mitsuhiro Kinoshita

Faculty of Pharmaceutical Sciences, Kinki University,

Kowakae 3-4-1, Higashi-Osaka 577, Japan

Analytical Biochemistry 297, 111±116 (2001)

doi:10.1006/abio.2001.5309, available online at http://www.idealibrary.com on

Find in attachment, a paper on: Peptids that mimic glycosaminoglycans:

high-a¤nity ligands for a hyaluronan binding domain

Michael R. Ziebell a;b; 1, Zhan-Gong Zhao b, Bai Luo c, Yi Luo b, Eva A. Turley d,

Glenn D. Prestwich

Chemistry & Biology 8 (2001) 1081^1094