How to ascertain using Mitochondrial DNA barcode similarity the relationship of the two mollusk species.?

21 April 2024 1 10K Report

Is their specific threshold for discriminating species similarity based on Mitochondrial DNA Percent similarity Identification particularly for Mollusk? What is specific DNA barcode (BLAST) Threshold or standard similarity to ascertain if the two species belongs to the same genus or if they belong to similar or different species?

Rodulf Anthony Balisco

Hebert et al. (2003) established a 2% threshold for intraspecific variations. However, they used many different taxa in their study. Based on my opinion, the threshold should be group-specific since 2% does not work in other taxa.

Similarly, do not just rely on molecular sequences for species delimitation and better to integrate it with morphological and ecological data.

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