Is it possible to chemically link a small peptide to the side chain of a Heparin sulfate proteoglycan? I know there are antibodies available, but I am specifically looking for a small peptide with less than 10 amino acids.
It probably is possible, but good luck documenting it. The Heparin sulfate molecule is an extended mess of ill-defined and ill-ordered sulfo-glycans strung on a protein core. Its best use is as a bulk molecule for taking up space. Son't waste your time getting into its fine structure - there really isn't any. Just attempt you linkage, and then see if the product has immune properties that are helpful to you.
Hi Tanveer, this may be possible using amine free peptide to conjugate carboxylic acid/sulphonic acid group of heparin using carbodiimide such as 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide.
Well. Tanveer, there you have it! A chemist's method to do the linkage. Let me know how you propose to separate the reaction product from the reagents.
Dialysis method will be good enough to purify the conjugated heparin from unreacted peptide or side products. All the best
Thanks Manohar, but I don't see the reaction between the peptide and carbodiimide. Can you please elaborate that.
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