Hi, In non-small cell carcinoma lung if IHC shows positivity for p40, p63, TTF-1, squamous and adenocarcinoma markers how to word the diagnosis?

In treatment of non-small cell carcinoma differentiating adeno from squamous cell carcinoma is essential.When morphological diagnosis is difficult IHC is done and sometimes we get adeno and...

14 September 2016 6,385 4 View

What are the possible statistical approaches that can be applied in a seasonal study on bird diversity?

15 July 2016 1,222 11 View

How does Artificial Neural Networks (ANNs) works actually in the identification of a species?

22 December 2015 3,672 4 View

Has anybody experienced a change in Eigen values and in triplot while calculating the CCA using PAST v.3.2 with the same data for a re-calculation?

Dear Researchers, I tried to plot CCA triplot using PAST v.3.2 with the same data set for a second calculation. & I experienced a difference in triplot and also in the eigen values. In the first...

01 January 1970 6,347 3 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View

I want to remove TiO2 and SiO2 coating from surface. any one can help me?

I want to remove TiO2 and SiO2 coating from surface. I think that i should use HCl and MgCl2 and an oxidation agent such as potassium nitrate, but i dont know in which propertion should these...

14 February 2021 300 3 View

References for relative humidity created by salt solutions at different temperatures?

I understand that the ability of standard salt solutions commonly used to produce a certain relative humidity (RH) in a closed space changes somewhat with the temperature. Where do I find data...

04 February 2021 7,370 4 View

For a PCR the volume of each component is standard ?

for example the total volume is 50microliters how can we know that what will be the amount of template , buffer, enzyme, primers (forward and reverse), dNTPs, MgCl2 and water?

02 January 2021 3,352 6 View

What's the function of each ingredient in a binding buffer?

I read a binding buffer in references. The recipe was listed as follows: Glucose (4.5g/L), Magnesium Chloride (5 mM), yeast tRNA (0.1 mg/ml) and BSA (1 mg/ml) was dissolved in DPBS. This buffer...

23 December 2020 8,440 4 View

How should I prepare solutions of divalent metals at neutral pH?

Hello, I would like to prepare metal solutions of 50 mM Mg, Zn, Ca, and Cu (separately) from their chloride salts, to study protein-metal-ion interaction. For this reason, I would prefer that it...

25 November 2020 1,924 11 View

What is the process to successfully cold draw Magnesium Alloys?

We have currently extruded Magnesium alloy AZ61A into Rods of 9.5mm diameter and are aiming to cold draw them to smaller sizes of 9mm, 4.5mm and 3mm. Can someone outline the cold drawing process...

24 November 2020 5,689 2 View

Smering in Pcr Amplification ?

I am facing a problem regarding smearing in PCR amplification since two months. But, before six months the results was there using the same primers at 67.2-degree celsius annealing temperature, 5%...

23 November 2020 4,394 5 View