In drug - DNA binding studies .........
(Ea-Ef)/(Eb-Ef) = {B - (B2 - K2 C [DNA]/s)1/2 } / 2KC
where Ea is the extinction coefficient observed for the absorption band at a given DNA concentration, Ef is the extinction coefficient of the complexes in its free form and Eb of the complex in the fully bound form.
K is the binding constant
C is the concentration of drug
s is binding site size
and
B = 1 + KC + K [DNA] / 2s
Ataf Ali Altaf
i am reviewing a research article, author of that article claim the utility of this equation in a wrong way, i think it is not possible to calculate K and s using these
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