My research aims to study Lectin-Carbohydrate interaction. However, I can't do the titration at the ITC, as I don't know the number of binding domains of the lectin.
Lectins are isolated from plants (by my research group) and carbohydrates are N-acetyl-D-Glucosamine.
Hello, you can take a look on the publications of Anne Imberty's group (cermav), they are doing a lot of ITC with lectins and carbohydrates. I will say from my experiences, the expected KD value is in high micromolar range. Even if you don't know the stochiometry of the binding, you can start with N=1.
Vanessa
Vanessa Porkolab Thank you very much for your referral from the researcher and help on where to start the experiment.
Note: I'll get back to you soon with news. =)
Check https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/lectin-binding
Kindly check this.
https://www.khanacademy.org/science/chemistry/chemical-equilibrium/equilibrium-constant/a/the-equilibrium-constant-k