Can I use a categorical covariate in a Generalized Linear Model in SPSS v.26?

Hello everyone, hope you are well. I am planning to use a Generalized Linear Model in SPSS. I would like to add gender to the model as a covariate, however from what I have read, only continuous...

14 February 2021 8,937 4 View

One-Way or Two-Way ANOVA?

Dear, In the preparations for publishing a manuscript, we have a big argument about the proper analysis of variance to compare multiple groups for a certain staining quantification. We have two...

27 December 2020 7,916 3 View

CD38 KO in MM cell lines?

Hi! I have been looking for publications where CD38 knock-out (or even knock-down) in multiple myeloma cell lines has been generated, but I have difficulty finding them. Have you come across...

29 October 2020 6,184 1 View

Is there a substitute to InhibitEX buffer in Qiagen Stool DNA extraction Kit?

I have a DNA extraction kit and would like to know if there is a way to make my own InhibitEX buffer (or something similar with the same function) to remove PCR inhibitors commonly present in...

23 August 2020 3,079 2 View

I need to perform antioxidant testings on Histocyctic lymphoma cells, any suggestons of another cell line, if it doesn't become available?

I need to perform antioxidant testings on Histocyctic lymphoma cells, any suggestons of another cell line, if it doesn't become available?

02 August 2020 4,502 2 View

As an academic researcher in Arab universities, what is your impression about the experience of distance education in light of the Corona 2020 ????

As an academic researcher in Arab universities, what is your impression about the experience of distance education in light of the Corona 2020 ????

04 May 2020 3,099 3 View

Can PLAXIS 3D use to evaluate the liquefaction analysis?

Dear researchers, I would like to know the ability of PLAXIS 3D, which can use to evaluate the liquefaction analysis or not? If can, could you please provide me links of its relating tutorials...

25 July 2019 7,058 3 View

DNA extraction from dry sealed tissue?

Hi, my colleague stored his samples of Drosophila pupae in PCR plate. He just put samples in wells, sealed it with PCR foil and left in room temperature for couple of months. He did not add any...

14 April 2019 3,282 2 View

What can be the potential applications of alloy powders?

I want to complete my research work by studying properties and applications of alloy powders.

17 April 2018 5,529 5 View

Any suggestions for desalting tips (or columns) to purify protein samples before gel top analysis (looking for high cut-off products 100-200 KD)?

high salt concentration in my sample is causing smearing in SDS-PAGE with no clear bands

21 November 2016 7,950 7 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View