Is it possible to buy normal plasma cells from healthy donors or RNA at least?

More Hana Svachova's questions See All

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View

Can I transfer the 3D file from Matlab to Ansys or Abaqus?

I have a 3D output in the figure section of Matlab, does anyone know a way to transfer it to Abaqus or Ansys? Or in what format should I save it so that these analysis software can analyze it?

09 July 2024 9,226 3 View

DNA extraction from clinical sample of Staphylococcus aureus?

Salam everyone, So I am trying to extract DNA from isolates of clinical samples of staphylococcus aureus, I am using a QIAamp UCP kit. I followed the same manufacturer protocol and used Qubit to...

03 July 2024 3,892 1 View

Plant extract sterilization???

Dear researchers, I've been working on aqueous plant extract sample, then I autoclaved them in 10 min to sterilize and added some preservatives. However, some of them showed bacterial and fungal...

11 June 2024 1,040 2 View

Editora Artemis, Editorial Académica Española: Predatory publishers?

Hello, On the basis of the conferences I have attended, I was contacted first by Editorial Académica Española to publish a book with them (a development of a conference paper), so I would like...

09 April 2024 1,751 3 View

How to eliminate a recurring mold contamination in the CO2 incubators?

Dear researchers, We are struggling with neverending mold contamination in all of our CO2 incubators. In the past, we used to do H2O2 decontamination/24hr-UV mode every two months now we do it...

04 January 2024 3,717 4 View

How can we improve the sonication process to achieve homogenous fibrils of the desired length (<= 50 nm) as indicated by Dynamic Light Scattering?

We conducted Dynamic Light Scattering (DLS) before and after sonication of our preformed fibrils of alpha-synuclein (aSYN) using a microtip sonicator. Our protocol was as follows: Amplitude:...

10 December 2023 2,768 1 View

How to statistically analyze species diversity more than two communities?

Dear coluegues, I want to analyze, parkland woody species diversity along agro ecology (low-land, midland & highland). how can I check if there is a significant difference along agro ecology...

06 October 2023 9,369 1 View

What are the problems of separating imprisonment and what are the solutions?

21 September 2023 299 0 View

Can I create a new sort of Research Item?

Hi, I would like to create a new sort of Research Item (namely workshops, some with video links). Is that possible? Or it should be better included in "Presentation"? Thank you, Hana

21 September 2023 8,926 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Michael J White

https://www.oxfordbiobank.org.uk/for-researchers/

Santhosh Arul

You may get plasma from healthy volunteers, however you require ethical approvals I believe.

Mothana Ali Khalil

you require ethical approvals to get it

Nishad Nawaz

Interesting question and looking forward to read answers