How can I connect HC-05 to epoc x ( EEG ) sensor ?

Greetings , my name is Abdul and I have this research on how to move a module car by brain wave . The EEG sensor I have is emotive epoc x , I did the data analysis and am ready to move the car but...

11 January 2021 7,204 1 View

Is it possible to use 100% Ethanol as mobile phase for C18 or any reversed phase columns?

I want to separate 2 compounds on HPLC, and I have made the preliminary studies on TLC using reversed phase plates and 100% ethanol as mobile phase which achieved complete separation. Now I want...

13 August 2020 2,229 3 View

Why trans protons and antiperiplanar protons have higher coupling constants in HNMR?

Why in HNMR the coupling constant of trans protons (typically ~17Hz) is higher than the cis protons (typically ~10Hz)? Also, why the antiperiplanar protons in a cyclohexane derivative have higher...

11 June 2020 4,731 4 View

Can I proceed Heck coupling on a compound in the salt form?

Can I do heck coupling reaction on a compound in salt form without turning it into a free base? As for example doing Heck coupling on p-chloropyridiniumchlroide. I don't want to turn my compound...

19 April 2020 5,593 12 View

Is 0.5 ml of DMSO enough to react my (200mg) Ar-Cl with 1.2 equivalent of primary amine using Ullman reaction?

I'm trying to react Ar-CL compound (200mg) with a primary amine (1.2 equivalent), and I've found in the literature that they're using a very low amount of solvents (like 0.5ml) to proceed the...

17 March 2020 1,016 5 View

Can I proceed N-Boc protection for an organic compound which is in the di-phosphate salt form?

My di-phosphate salt compound contains two 3ry amino groups involved in salt formation and a one 2ry amine group that's not involved in salt formation. I wanna proceed N-Boc protection for this...

15 March 2020 945 3 View

My supervisor told me my question and hypothesis are broad and too obvious, what should I do I already finished writing?

So I am writing about hate speech and chose two case studies from Denmark and France. And what I found is that hate speech is heavily influenced by nationalism but my supervisor told it is broad...

03 March 2020 658 3 View

How can I evaporate Methanol from extract by water bath as an alternative of rotavap ?

I have 10 ml methanol extract and unfortunately, my lab is lack a rotavap how can I evaporate this solvent by water bath

16 January 2020 4,897 2 View

HPLC actinomyceyes active compound ?

I have actinomycetes extraction and I need to detected by HPLC, so I need the protocol of hplc RP-8 chromatographic column

29 December 2019 7,578 3 View

Probelm with expressing hetrologous proteins using Pichia pastoris?

07 December 2019 7,502 6 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

What is the nutrient requirements for E.coli BL21 CodonPlu RIPL strain growing in M9 medium?

Hello. I want to isotopically label the my protein, and I express it in BL21 RIPL using M9 minimal media. It grows extremely slow. I inoculate the colony from a fresh M9 plate into 5ml M9 medium...

17 February 2021 2,852 2 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

Can I modify vermiculite or perlite by adding nutrients to control a certain situation?

I need to assimilate a determining condition for the plant. However, I need to ask you if it is possible to change the properties and concentration of plant nutrients in this material like perlite...

04 February 2021 8,616 11 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View

How can you adjust pH of SM buffer?

Good afternoon, please how can you adjust pH of phage SM buffer? I presume that main ingredient that has influence of pH is Tris/HCl buffer, but according to literature its range of pH is from 7...

27 January 2021 5,767 3 View