Is it possible to assess serum expression of MMP2 and IFN gamma by western blotting?

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Can anyone help with the ebook on Environmental Encyclopaedia by Cunningham, Cooper, Gorhani, Hepworth, Jaico Publishing House, Mumbai ?

Can anyone help with the ebook on Environmental Encyclopaedia by Cunningham, Cooper, Gorhani, Hepworth, Jaico Publishing House, Mumbai?

12 May 2024 5,684 0 View

Why is RNP-RNA migrating faster than free RNA in EMSA gel at higher protein concentration?

Hello everyone, I have performed EMSA experiment with an RNA-binding protein and RNA. For both ssRNA and dsRNA, for higher concentration of protein, I observe a high intensity band at the dye...

18 April 2024 10,034 1 View

Can someone suggest me a possible method to prevent activity loss of vitamin D3 over the time?

I am working with vitamin D3 and every time wea re facing problems with storage and lost activity of vitamin D3. We had tried every possible method such as working in low light, storing at...

29 March 2024 5,415 0 View

Unable to upload research article in pdf format. what is the process?

parser server error

20 March 2024 4,542 0 View

Can anyone suggest/inform about a free software for drawing scientific illustrations, except BioRender?

Thanks in advance!

02 February 2024 9,846 1 View

Genome wide CRISPR screen?

Hi All, I need an opinion about an experiment I am planning to do. I am planning to do a genome wide CRISPR/Cas9 screening to identify genes responsible for drug resistance. I have purchased the...

02 January 2024 6,369 2 View

Can anyone suggest me the solubility of aspartame in water or buffer as it can be used in cell culture?

I am trying to make 250 mM Aspartame (CAS RN: 22839-47-0) and used autoclaved dist. water as solvent. But after trying with water , cell culture grade ethanol and DMSO with different...

08 December 2023 5,153 1 View

Can a fto electrode be reused for second time? If yes how can we clean it before reusing it?

I've got a new fto electrode and did my testing with a copolymer material. Now I have another material ready so can i reuse the fto electrode for the second time. If yes how to clean and reuse it.

04 December 2023 5,964 1 View

Is there any scientific software which can generate 3D model structures of self-assemblies on command?

I am looking for a paid/open-source software which can generate 3D model structures of self-assemblies (like vesicles, nanotubes, fibrils and other 3D structures) on command. I have attached an...

25 October 2023 9,497 4 View

How is steam jet ejector added in Aspen Plus?

Steam jet ejectors are not present in the built in models of Aspen Plus. How to add a steam jet ejector in Aspen Plus. Can it be done by the combination of two models or are there any other...

18 October 2023 5,200 1 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

Culture fibroblasts with or without FGF2?

I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...

29 July 2024 3,030 2 View

What will be the best way to test NFkb activation via western blot?

Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?

18 July 2024 5,214 4 View

ELISA sandwich with two monoclonal antibodies ?

hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...

16 July 2024 9,044 10 View

How to detect phosphorylation on Western Blots?

Having trouble detecting phosphorylated HSL. Any tips for how to prevent de-phosphorylation events or detect phosphorylation?

15 July 2024 2,055 3 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

Why is the baseline fibrosis level of HK-2 cells very high?

In many experiments where HK-2 cells were stimulated with TGF-β at 10 ng/mL for 48 hours, I observed that even in the control group, the baseline expression level of fibrosis proteins like FN1 and...

15 July 2024 1,929 2 View

Is there anyway to distinguish two proteins of same molecular weight?

I know mass spectrometry will work. But, is there any simple and straightforward method to distinguish between the two proteins of the same molecular weight? They are cryptochromes and...

14 July 2024 9,324 2 View