I need to analyze protein-functionalized graphene in a water medium by Raman spectroscopy.
Yes. There is no interference from water in Raman spectrum. Water is Raman inactive.
Thank you. A question: how you can perform the Raman analysis? You put a drop on graphene and than you focalize? I use graphene on solid substrate like glass, or silicon etc....so I need to know the procedure. Please, tell me the procedure to measure.
Thank you in advance.
You may directly perform the Raman by putting a water droplet on your sample if the objective could work in water medium. Or you may put a glass above the droplet and try to use an objective with long working distance. We do research on electrochemical Raman (in aqueous solution) and that is the way we adopt.
@Prompong Pienpinijtham: I wouldn't say that water is Raman inactive. :-)
You can find a weak H2O deformation signal at around 1750 cm-1 and three broad signals from OH vibrations in the range of 3000 to 3750cm-1. Just that you are not surprised to find signals at these positions.
But depending on your setup and integration time, those signals will be hard to detect. I expect the protein and graphene signals to be of much higher intensity compared to the d(H2O) and v(OH) signals.
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