Is it possible that adenovectors change the conformation of the cloned protein when its expressed ?

More Hamsa Jameel Banjer's questions See All

Can you provide me with the best p16/ki67 dual staining protocol for FFPE tissue (cervical)? and what are the best antibody brand for that?

can you provide me with the best p16/ki67 dual staining protocol for FFPE tissue (cervical)? and what are the best antibody brand for that?

19 December 2023 3,957 1 View

Inquiry about sleeping quality scale for free?

I want to know a scale measure sleeping quality but need no fees to use it and I want it to be available in Arabic version so can anyone help to recommend me a sleeping quality scale that is for...

03 November 2023 6,301 0 View

What are the post harvest treatments that can be followed to reduce fungal infection of carrots, cauliflowers? in need of treatments recommendation?

Facing issues with rottening of carrots and cauliflower, perticularly during rainy season After two days of harvesting facing the issues. What might be the problem n need of treatments recommendation

13 September 2022 1,691 0 View

GWAS Public Portal for diabetic and dialysis patient?

Is there any public databases or projects portals of DNA-Seq, RNA-Seq or GWAS data available online for diabetic and/or dialysis patient, or for microbial/fungal susceptible individuals ?

01 September 2022 8,236 0 View

How should i represent my GSEA of pathways, by the ES or by the NES?

mathematically and statistically i don't real understand what is the difference between the ES and the NES, and i want to present my result in a bar graph, so should i use the ES or the NES?

01 February 2018 3,656 1 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

Angel A. Rivera

Can you detect protein amount by ELSA?

Can you do immunofluorescence or FACS analysis ?

Please be sure that you have a protein control in your WB to be sure that your reactions are working well.

Hamsa Jameel Banjer

I can detect it by immunofluorescence. But anyway, now I'm thinking that my problem was the denaturing of the protein before loading the wb gel, last time I added DTT and I think I can see a very light band.