I want to synthesis carbon dots to specific binding of E.coli, so i modified with mannose because i know after i read some paper mannose only selectively labeled E.coli but now after modified it. I try to binding of E. coli and also staphylococcus aureus to know, its specific or not for E.coli and i get my carbon dots not specific for E.coli, because also get flourescence intensity for Staphylococcus aureus. and then i also check it by use FTIR to see mannose was successfully or not modified on carbon dots. the result is mannose modified in the surface of carbon dots. So what's should i do and what's is the problem in here?
Carbon dots are extremely small and can penetrate cell wall and enter cytoplasm. Probably that is the reason that you do not have selectivity over a specific bacterial species. You should consider functionalization of carbon dots with a molecule that binds to cell wall receptors that E.coli have in a higher abundance, compared to S. aureus
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