Hi, it might be possible but you have to deliver the plasmid to the cell and this could be by bombardment or by protoplast transformation but to prepare the protoplast is not easy task. Also RNA pol II recognize 35S in plant cells.

The circular plasmids can be expressed in plant cells but the gene of interest has to be under the control of appropriate promoter adn you need to deliver the plamsid to the cell. In fact whe you use protoplast transformation or bombardement you use circular plasmids. Why don't you use Agrobacterium mediated transient expression? I don t know which species you are using but it is working quite well for me.Good luck!

Thanks a lot  Yordan

You're welcome Zahra!

Hi Zahra,

Yes, pBI121 can express gene-of-interest without using Agrobacterium. Two examples are here:

(1) pBI121 expression using particle bombardment in plant cells (see attached paper #1).

(2) pBI121 expression through PEG-mediated genetic transformation in rice plant cells (see attached paper #2).

However, I don't know if you are aware of a technology called 'Agroinfiltration'. For transient assay, actually Agroinfiltration is a fairly easy technology. The steps include cloning your gene into a binary vector, transferred the vector into Agrobacterium, inject the culture of Agrobacterium into for example tobacco leaves, and after 1 or 2 days you can transiently assay your gene product. It doesn't need really fancy devices to conduct. Bombardment needs a gene-gun machine. Protoplast transformation need to prepare the cell-wall protoplast, a tedious work. Besides, the binary vector (with T-DNA elements) are suitable for using in particle bombardment and PEG-mediated protoplast genetic transformation in the future, if needed.

Best,

Hi Yuan-Yue and Thanks a lot

I agree the answers above. You might also can try nano particles as delivery vehicle.

Hi Wan-jun,

Thank you for your attention.

Do you know which type of nanoparticles and nanocapsules can be used for gene transfer in plants? On the other hand,which type of polymers can be used to make nanocapsules  that can pass through cell wall and conjugate in plasma membrane?

thanks

Hi, Zahra,

Materials is out of my knowledge. Might be silicon... You can read the following papers:

He B., Chu Y., Yin M.*, Müllen K., An C.*, Shen J.* Fluorescent Nanoparticle Delivered dsRNA toward Genetic Control of Insect Pest. Advanced Materials. 2013; 25, 4580–4584. 

Jiang L., Ding L., He B., Shen J.*, Xu Z., Yin M.*, Zhang X.*. Systemic Gene Silencing in Plant Triggered by Fluorescent Nanoparticle Delivered Double-Stranded RNA. Nanoscale 2014; 

Chang et al. A simple plant gene delivery system using mesoporous silica nanoparticles as carriers J. Mater. Chem. B, 2013, 1, 5279

Thanks a lot Wan-jun

It will definitely express, and may also integrate randomly