What is the proper range of FRET efficacy?

I would like to use FRET effect to probe the distance between two functional moieties (I am sure the distance is smaller than 10 nm). I have tried several times, but I found out that the FRET...

21 February 2021 3,562 1 View

What's the function of each ingredient in a binding buffer?

I read a binding buffer in references. The recipe was listed as follows: Glucose (4.5g/L), Magnesium Chloride (5 mM), yeast tRNA (0.1 mg/ml) and BSA (1 mg/ml) was dissolved in DPBS. This buffer...

23 December 2020 8,440 4 View

Why transwell insert can cut off some drug molecules?

I am preparing a co-culture experiment in which transwell inserts will be used. According to the references, 0.4 micrometer PET membrane was selected. And different drugs were added to the upper...

15 November 2020 3,718 2 View

Can I use HUVEC cell line, not primary cell, to form barrier system in transwell?

I would like to measure the permeability of my drug using in vitro model, transwell, specifically. However, I only have the cell line, not the primary cell. Can I use the cell line to plate in the...

08 November 2020 3,330 1 View

How can I choose the concentration of Triton X-100 when I would like to permeabilize cells for flow cytometry?

Triton X-100 has been widely used to permeablize cells for flow cytometry. How can I choose the concentration of Triton X-100 if I would like to detect expression of one protein in the whole cell?...

31 October 2020 462 1 View

How to measure the treatment effects in RCT design with multiple baseline and end line rounds?

I'm working on a RCTs study on the impact of nutrition training on crop diversity of small farmers. We will train them on nutrition and observe the changes in crop diversity and their diet. We do...

27 September 2020 8,081 3 View

Could anyone help me to determine the structure of this molecule?

Dear all, I need to determine the structure of the following molecule, see the addached file, could anyone help me? Thanks very much!!!

22 June 2020 9,128 1 View

How to convert data rate to reliable communication?

Dear all, I have a stupid question as follow: I read some papers related to the comparison between the capacity region between some types of wireless Medium Access Control (MAC) such as TDMA,...

22 May 2020 6,668 6 View

Dose someone could give me sugestions about 3DNA, Nucleic acid builder, Namot2 and PyMol?

I am working on the MD simulation of 3D self-assembling nanostructures. But researchers in our group have mainly worked on proteins, small molecules and synthesized polymers. Softwares including...

31 March 2020 9,688 3 View

How to chose right data form Protein data bank?

I would like to make MD simulation of a 42-bp B-DNA double helix, which has a segment of single strand at each end of the chain, and a small molecule anti-cancer drug. I have searched PDB and NDB...

09 March 2020 3,605 3 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View