Is it possible a good protein extract (including extracellular factors) after intra-cardiac vascular washing with saline/PBS?

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How can I identify the points that are poking out from a point cloud plot?

I have a cloud plot of data relating two important variables for an ecological analysis. I was defining the "abnormal class" as the data points that are >=20 for the Y-axis and >=20 for the...

22 September 2020 7,679 16 View

Why is the pressure slowly increasing when using a Hiplex Ca column?

We recently installed a Hiplex Ca Duo Column from agilent for carbohydrate determination. It was working fine until a week ago, when the pressure of the column started to increase very slowly. As...

03 September 2020 1,733 2 View

How do I detect primer dimers in an electrophoresis gel? and how to avoid them in a PCR reaction?

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04 May 2020 7,455 6 View

All-against-all pairwise global alignments?

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28 March 2020 8,869 3 View

What Does Saint Thomas Say About Immigration?

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2D-NMR DOSY peak intensities, what does that mean?

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No title

The effect of ENSO on weed populations dynamics is of key importance for better weed management or species conservation purposes.

31 January 2020 1,673 3 View

Is there a recommended article on RAMAN Spectroscopy and/or FTIR that also works as a guide?

Fellow scientists, I'd like to know if a test solution contains certain leachates, meaning that an extraction method I am developing works. Much appreciated beforehand.

13 January 2020 5,249 3 View

What is important to consider in an antibody titration, volume or concentration? Is it needed combined titration of Abs when they are used together?

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Digesting bioplastcs (PLA)?

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MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

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How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

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How to quantitative analize the data of TNBS assay for gelatin?

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02 March 2021 2,616 1 View

How to repeat an operation on an atom selection for multiple times in PyMol?

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Jesus M Grondona

Perdona que te responda en castellano... El grupo de Antonia Gutierrez lo hace... Les puedes preguntar.

David Baglietto-Vargas

You can perfuse the mice 3 min with sterile PBS. Following, half of the brain can go in 4%PFA and the other half you can freeze in dry ice.

Manuel F López-Aranda

I recommend to perfuse the mice for 5 minutes with cold sterile PBS. Once you extract the brain put the half on 4% PFA overnight and the other half you can freeze in dry ice.