Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

How or where can I get the crystallographic information file (CIF) for PCN-222 MOF?

I need the simulated XRD pattern of PCN-222 to compare with experimental data. Thanks.

19 February 2021 4,932 3 View

Websites for virtual lab in semiconductors?

Hello everyone, Because of Covid, now all classes are online. Is there good website that also are easy to use to teach student virtual experiments about PN junctions and semiconductors? Thank...

24 December 2020 6,637 2 View

From the EDAX analyses if the material which is coated is not observed in maximum percentage then how can interpret the results?

If we doped metal oxide to semiconducting material in different ratios then the property of the resultant material will be increased.But in some lower ratios when we go through the elemental...

16 December 2020 8,190 2 View

Is there any software for calculating hit bias (HB), missed precipitation (MP), and false precipitation (FP)?

I have a time series of two rainfall products. For error decomposition analysis, I have to calculate HB, MB and FB. Is there any software for calculating these variables.

27 November 2020 980 2 View

Why Schiff bases change colour upon evaporation?

I got bright yellow coloured Schiff base after 6 hour reaction time. So i kept it for slow evaporation. Solvent was methanol. But after complete evaporation my product colour changes from yellow...

17 November 2020 3,824 2 View

How to make the children cool, who feel stressful during this pandemic-lock down without having physical outlet?

The discussion may feed the parents a way to manage and guide the children in a right direction.

12 November 2020 1,302 9 View

How to design qPCR primer for mature microRNA?

09 November 2020 9,163 3 View

Which force field should be used to simulate protein-protein complex in gromacs?

Hi, I have done a protein-protein docking. Now I want to perform MD in gromacs. I am confused with forcefields. When I tried to findout, everywhere it is written for protein-ligand but I couldn't...

29 September 2020 1,410 3 View

How to purify Plasmid DNA after RNAse treatments and Restriction enzyme digestion for high yield Capped RNA Transcription ?

I tried phenol/chloroform purification method with 2 microgram DNA after RNAse and Restriction enzyme digestion but I am not getting enough quantity of plasmid DNA at the end. I am just getting...

25 September 2020 7,591 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

What is the nutrient requirements for E.coli BL21 CodonPlu RIPL strain growing in M9 medium?

Hello. I want to isotopically label the my protein, and I express it in BL21 RIPL using M9 minimal media. It grows extremely slow. I inoculate the colony from a fresh M9 plate into 5ml M9 medium...

17 February 2021 2,852 2 View

Where would? can? positive charge(vacancy) be when grounded?

Hello I plasma(H2N2) treated Al2O3 plate (edge is connected to ground) and wondered if oxygen vacancy migration on the plate can occurs as electrons escape through edge. Or whatever positive...

15 February 2021 8,118 1 View

ZnS oxidizes to ZnO!!why??

when I put my ZnS solution in the chemical bath, the ZnS oxidizes so there are ZnO peaks appearing because of the reaction with the oxygen in the air, despite the fact that I close the beaker...

14 February 2021 5,778 9 View

How to prepare OH- terminated Si Wafer?

Hi, Folks. I am working on the ALD project to deposit Al2O3 on the Si wafer from TMA and Water. I have struggled with the preparation of OH- terminated Si wafer. I have both Si(1 0 0) and...

08 February 2021 8,792 2 View

Can I modify vermiculite or perlite by adding nutrients to control a certain situation?

I need to assimilate a determining condition for the plant. However, I need to ask you if it is possible to change the properties and concentration of plant nutrients in this material like perlite...

04 February 2021 8,616 11 View

Is it possible to have a diffusion of oxygen from the glass substrate (SiO2) into the absorbent layer during annealing process?

02 February 2021 382 7 View

How to do ORR test with pine(Which means RDE or RRDE electrode)?

I want to do LSV ,CV test of ORR(Oxygen reduction reaction). But our laboratory don't have the RDE and RRDE electode. And I was wondering if I can do the tests by magnetic stiring or mechanical...

14 January 2021 1,869 2 View

How is oxygen vacancy formed in Tio2 and help with anatase to rutile transformation?

I am unable to visualize the basic formation of Oxygen vacancies in Tio2 as sometimes information is a bit scattered. Can anyone help me with how these point defects are formed and what prompts ?...

08 January 2021 2,017 4 View

Can you give me some Information about the generation of ROS in marine surface water?

Reactive oxygen species is a very new term i think

23 December 2020 8,416 4 View