Is it okay to have Ct value for target gene less than house keeping gene?

H Surachandra Singha @H-Surachandra-Singha

11 April 2019 1 5K Report

I am getting Ct value for target gene less than the house keeping gene. Now the problem is i am getting negative values in delta Ct value. So is mandatory to subtract the reference Ct value from the target one or it okay to alternate the position.

Patrick Pascal Lehr

Hello H Surachandra Singha, did you find an answer of your question?

Thank you in advance?

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