H Surachandra Singha

5 Questions 7 Answers 0 Followers

Questions related from H Surachandra Singha

How to construct Scatter plot for KEGG or GO classification visualization?

Can anybody tell me how to construct this scatter plot for KEGG or GO classification like the image I have attached?

28 September 2020 5,640 3 View

What is significant Fst value range for SNP analysis?

I have performed an SNP analysis of the whole transciptome data. Now I have received Fst values ranging from 0.3 to 0.9. Can I consider the SNPs with Fst value above 0.5?

07 July 2020 9,889 4 View

Is it okay to have Ct value for target gene less than house keeping gene?

I am getting Ct value for target gene less than the house keeping gene. Now the problem is i am getting negative values in delta Ct value. So is mandatory to subtract the reference Ct value from...

11 April 2019 4,785 1 View

Why my RNA extract is showing degraded?

I have preserved my tissue sample (ovary and follicles) in RNA Later and stored at -80 degree celsius. I have tried to extract the RNA using Trizol reagent. I have even washed the tissue with 1X...

07 January 2018 8,744 18 View

How to collect hypothalamus?

I am looking for a good procedure to collect the hypothalamus from an avian brain without disturbing the form of the organ.

10 October 2017 6,583 1 View