Is it okay to dilute a bacterial culture with LB to lower the concentration into the ideal range during IPTG induction?

15 September 2021 1 8K Report

I overgrew my bacteria according to OD. I just removed the seemingly correct volume and replaced with LB to get into the ideal density. I then added IPTG..Should this work?

Hassan Nasser

Dear Neal:

You could benefit from this valuable link:

https://www.researchgate.net/topic/Isopropyl-b-D-1-thiogalactopyranoside.

I hope it will be helpful...

With my best regards ...

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