Does anyone know of a more affordable alternative to Mito-Tracker, that is well retained in fixed cells?

I have in the past worked with the MitoTracker dies. They are rather expensive and that is the bit I am hoping to avoid. I would really appreciate it if anybody can suggest a mitochondrial...

05 November 2020 5,701 1 View

What is a good ligation strategy with compatible ends EcoRI and MfeI in the vector DNA and the insert?

I used to do this kind of thing and now have forgotten strategy please help and be specific. The vector is now linear having been digested with EcoRI and MfeI, then treated with CIAP, to prevent...

23 May 2020 2,377 1 View

Could anyone send me a FileMaker template to create a biological collection (bacterial strains, mutants, etc)?

I am planning to create biological databases for yeast, bacterial, strains and plasmids. I want to use FileMaker pro but I am a new user/ A template would be of great help to see how I can modify...

20 October 2018 6,708 1 View

Can I set up a plate for qPCR using Power SYBR Green PCR Master Mix #4367659 and run it a day later?

Catalog number: 4367659 Power SYBR Green PCR Master Mix. This is NOT POWER UP that I am asking about. Can I set up a plate Monday the 28th in the afternoon and run it Tuesday the 28th in...

24 May 2018 8,588 5 View

Does anyone have a tried and true protocol for transfecting RAW cells with plasmid DNA for protein expression?

02 June 2016 7,457 0 View

Does anyone have a complete protocol for generating total cell lysate for proteomic analysis from adherent tissue cultured cells?

04 May 2016 3,844 2 View

How can I image live cells in 3D systems and subsequently maintain them in culture?

I am growing bladder cancer cells in 3D models in vitro and I wish to visualize them using some form of real-time imaging which will still allow me to grow them and use them for protein/RNA...

11 May 2014 853 7 View

*CRY file for Molybdenum

Does anyone have a *CRY file for molybdenum or know where I can get one from the web, please. I am trying to EBSD a moly, titanium, iron alloy.

01 January 1970 5,127 3 View

Is there a protocol to establish a stably transfected macrophage cell line, preferably in mouse?

01 January 1970 5,394 1 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Any easy method of analyzing vitamin D in foods by HPLC?

Looking for method of extracting and analyzing vitamin D in foods by HPLC

24 February 2021 7,524 7 View

Trouble shooting with his tagged protein expression in E.Coli?

i have issues in expressing a chicken protein in BL21 cells with his tag. I do not see induction even at 18 degrees. Any suggestions

15 February 2021 8,939 3 View

Why the storage moduli E' decrease with highr fiber volume?

Hi guys, I am facing problems to interpret a DMA results. Material: Composite Bisphenol A + curaua fiber What is being tested: the influence of fiber volume on the composites properties...

12 February 2021 174 7 View

Troubleshooting with immunofluoresence/confocal microscope? What went wrong?

Hi guys, I have no experience and little knowledge on immunofluorescence and confocal microscopy. I tried to visualize the expression of FXR in my cells (THP-1 macrophages), yet I did not...

11 February 2021 562 6 View

XRPD Quantification - Building a calibration curve of drug excipient?

I am aiming to utilise XRPD to quantify a drug excipient within a drug product and have been having a little trouble building the calibration curve to allow for this quantification up until this...

10 February 2021 6,145 3 View

Identical spectra for L- and D-peptides after they self-assemble?

Hi dear all, I need advice. I have two peptides with the same sequence but one consists of L-amino acids only and the other consists of D-amino acids only. when they self-assemble in aqueous...

03 February 2021 2,032 3 View

What is the best concentration of Pam3CSK4 to coat a dish to activate with THP-1?

Hi everyone I am trying to coat a glass-bottom dish with Pam3CSK4 and plate THP-1 cells to measure IL6 with ELISA from the supernatant. I wondered if anyone knows a good concentration and...

21 January 2021 2,593 1 View

Can you polarize Jurkat cells into different T helper subsets?

I am wondering if anyone has had success with in vitro polarization (via cytokines) of Jurkat cells. My goal is to understand the affect of a drug on Treg induction and differentiation. I...

12 January 2021 3,606 2 View

Can I use 24 wells black tissue culture uncoated plate for cultuing macrophage (THP-1 treated with PMA) ?or I have to coat them?

Hello, I will start an experiment using macrophage (THP-1 treated with PMA). I have ordered the plate that suitable for fluorescence plate reader which is glass 24 wells black uncoated tissue...

05 January 2021 4,214 3 View