How to ensure a marker antibody won't activate T cells and affect the result?

I'm recently buying some anti-CD3 and anti-CD28 to activate T cells. I also notice some of this clones have been conjugated with fluorescence for the purpose of labeling T cells for flow...

09 October 2019 962 4 View

Why my gene in single-cell sequencing showed zero value?

I have many single-cell sequencing data. I checked my gene of interest in these data. However, all of these single-cell sequencing data showed that my gene has no expression, no matter in immune...

31 December 2018 3,638 3 View

Why can't I detect the surface protein by western blot?

I was doing an over-expression experiment of a gene for a year. It is a surface protein. The biggest trouble is that I can never detect the gene overexpression by western blot. Interestingly, when...

10 November 2018 7,195 3 View

Why some cancers are not prone to mutate?

When I first learn cancer biology, my teacher told me that cancer is usually caused by the loss of tumor suppressor , which is like you loss your brake. After it losses the suppressor, it tends...

05 June 2018 8,052 2 View

Are there any cancer cell lines that can form tumor in nude mice but not in normal (immune competent) mice?

Are there any mouse cancer cell lines that can form tumor in nude mice but not in normal (immune competent) mice? Please give me some examples. Thanks.

04 May 2018 6,666 2 View

What are the current applications of Artificial Intelligence in biology or cancer?

AI seems to be pretty popular these days and there are many applications in our daily life. But are there any AI designed to help our researchers to solve any biology questions?

04 May 2018 4,024 3 View

Why don't use ampicillin to select transduced mammalian cells?

Why don't use ampicillin to select transduced mammalian cells? also, I saw some lenti-vector is Bleo Resistant, is bleo used as bacteria selector or mammalian cells selector?

03 April 2018 7,544 0 View

Can I cut the two ends of a gene with same restriction site and clone it to a vector?

The two ends of my gene have same restriction sites. And the destination vector also has two same sites. Can I clone the gene directly to the destination vector? I know it's possible that the gene...

03 April 2018 8,318 4 View

What's the antibiotic cell selection for plasmid FUGW?

According to the website https://www.addgene.org/14883/, it has bleomycin resistance. However, bleomycin can be used both for bacteria selection and cell selection. Then what is the cell...

03 April 2018 3,768 1 View

For CRISPR, transfection or transduction?

For CRISPR, transfection or transduction? The CRISPR vectors I bought is lenti vector, so I can either do transfection or transduction. But which is better??

02 March 2018 2,193 4 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

How to get/count refractive index in gasses?

20 February 2021 8,202 6 View

How to make Fast Green FCF from powder?

Hi everyone We need Fast Green to electroporate plasmid into mice embryos, therefore we simply need it to see some color in the liquid we are electroporating so that electroporation is more...

07 February 2021 1,671 2 View

Which is the best software for the simulation of tribological properties of materials?

I want to simulate the tribological properties of solids, liquids, and fluids, please suggest good software.

02 February 2021 3,947 3 View

Does anybody work with CoolLED in fluorescence microscopy?

I have trouble with exciting wavelengths higher than 460 nm while e.g. ex with 405 nm works. Filtercubes are correc, as I used them with my old xenon lamp before... I work on axiovert200M and with...

25 January 2021 5,201 2 View

How to prepare a liquid sample for FTIR spectrum?

I have a ethanol-water 50% extract of mango peel. I purified mangiferin from the extract with filtration and precipitation. so i obtained a low concentrated extract which i want to se the IR...

24 January 2021 8,880 7 View

Why high voltage and current (and some gases) Cause Spray-mode metal transfer in Gas-Metal Arc Welding (GMAW)?

This transition phenomena of Globular to spray mode as voltage , and especially current is raised, is very commonly known to Manual Metal Arc Welding (MMAW) and Gas-Metal Arc Welding (GMAW)...

20 January 2021 7,984 2 View

Can you have pressure inlet and velocity inlet at the same time?

I was reading a reference (Reitz-A Photographic Study of Flash Boiling Atomization). In his experiment, his boundary condition is delta p and constant velocity. The liquid injection pressure was...

18 January 2021 842 9 View

How can you confirm new liquid FTIR cell fits calibration?

We have an FTIR instrument which was calibrated in transmission/absorption mode using a set of cells for different ranges of D2O concentration. One of the cells at high range broke and the new one...

16 January 2021 4,791 2 View

Where can I find these two papers?

14 January 2021 1,572 3 View