Hello,

For using ImageJ to analyse gel image, normally I select each lane for each rectangle/box. but some guides suggest to draw a rectangle around all the lanes of gel image in one box and then plot lane, which is easier by the way.

Is it ok to do that to summarize the data in publication?

For lane background subtraction,

which one is better to:

1) draw vertical line to cut/seperate peak from background (first figure)

2) draw line between end point of the peak (second figure)

Thank you very much

Cheerio,