How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

Mixed ANOVA or Multilevel modeling?

Hi everyone, I'm having some questions about choosing the right test for my experiment and I hope I can find help here! Here are the settings of the experiment: 1. participants are randomly...

14 February 2021 1,028 4 View

How to do variation partition for a distance matrix for microbial ecology?

I have bacterial, fungal, and protist OTU table. I want to know to what extent protist and fungi distribution can explain the bacteria distribution. I only know Mantel test could do a correlation...

11 January 2021 4,164 2 View

Are there any method to detect implanted stem cells?

Dear, We developed a microcarrier that was pre-seeded hUC-MSCs to repair a rat bone defect. However, we didn't use GFP or Luciferin to track cell survival. If there's any way to identify the...

15 December 2020 6,065 3 View

How to characterize the dispersion of aluminum oxide in Al matrix composite which reinforced by alumina during the powder metallurgy?

Now we could characterize the disperion of Al2O3 in AMCs (pure Al reinforced by Al2O3 ) after sintering. But the process of powder metallurgy has a lot of steps, which take long time. The cycle...

08 November 2020 5,244 3 View

Is that possible the filter of flow cytometry was failed so I got those data using FL1 vs FL2 and FL1 vs FL3?

The cells were expressing GFP and also incubated with PI for dead cells detection. This is my first-time using this BD Accuri C6 and I'm not sure if I need to set compensation (I tried to follow...

01 November 2020 8,360 3 View

What if the abbreviations of two technical terms in the manuscript are the same?

I'm wrting an article, there are two terms "Carboxymethylcellulose" and "Critical Micelle Concentratio" both have the abbreviations of "CMC". How should i distinguish them?

23 June 2020 2,115 5 View

ImzMLConverter installation & running issues?

Hi Everyone, Did anyone have a similar issue when install and run imzMLConverter? I followed the instruction on https://www.cs.bham.ac.uk/~ibs/imzMLConverter/ and used the command it provides....

23 May 2020 3,936 3 View

How to revise survey items based on unexpected EFA results?

Hi, there. A few days ago, I posed a question about factor analysis of survey results, but I left many important details unspecified. HERE is the more detailed one. I develop a theoretical model...

06 May 2020 4,937 10 View

Many cases of identical answers to consectutive questions.How to revise survey items?

Hi, there. I am working on my Ph.D dissertation. I have run a factor analysis of 56 items with maximum likelihood and direct oblimin method. It turns out that only one factor can be extracted...

25 April 2020 6,267 10 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

How two melting peaks in DSC of polypropylene can be merged into one crystallization peak?

The figure shows DSC cooling (blue) and 2nd heating (green) curves (step: 10 K/min) for a commercial sample supplied as Atactic Polypropylene (APP). The heating curve illustrates two major...

03 March 2021 5,857 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

What information can be had from raman at higher wavenumber?

i have recorded raman spectra of strontium oxide thin film. the spectra were recorded in the range 400 to 4000 cm-1. i have reference to peaks upto 1000cm-1. but i couldn't find literature for...

26 February 2021 2,499 1 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View