In ELISA should we use p-nitrophenyl phosphate (pNPP) substrate mixed in distilled water or we should mix it in Diethanolamine buffer?

We are performing an ELISA experiment to determine the binding of Complement antibodies against P. aeruginosa. We have pNPP substrate (Thermo Scientific cat # 34045) instead of pNPP tablets. We...

22 December 2020 2,020 3 View

I am working on research which will try to establish link between sentiments and psx returns (pakistan). Can anyone help me with it?

I am looking for data scraping step by step guide to scrape data from twitter accounts in Pakistan. I will be using top 100 accounts by following. Can someone guide how is this possible. I will...

10 August 2020 5,079 5 View

Can we add glucose for making M9 minimal media containing C6-HSLs as a sole source of carbon?

I am workimg on isolation of quorum quenching bacteria from soil. I want to use M9 minimal media containing homoserine lactones as a sole source of carbon. Therefore, can we add glucose too into...

14 October 2019 4,718 2 View

Abaqus Elastoplastic Analysis??

Hii, I am modeling Transmission tower (steel str.) using elastoplastic properties of steel. Type is dynamic Explicit (Earthquake load acting at base of tower). But while running the model i am...

12 September 2018 2,390 4 View

How to remove the wax covering layer of hemipterans ?

What's the best way? to remove the wax covering layer of hemipterans (such as citrus psyllids), for Scanning Electron Microscopy?

26 March 2018 5,495 4 View

How to get user manual or tutorial video of ATOM, JTEX and VPSC7?

I'm working on severe plastic deformation of face centered cubic materials and require these software's user manual for texture analysis.

13 December 2017 4,528 3 View

Does any researcher know the biochemical reaction of wheat straw and its biochar on soil nitrate (NO-3)?

In one of the experiment, I applied wheat straw and wheat biochar at three different level to the field. Soil NO-3 gradually decreased with the increasing level of Biochar while, wheat straw...

23 November 2017 7,852 4 View

Why lattice parameter first increase then decrease in case of doping Cu in zinc iron ferrite ?

I am working on spinnel ferrite synthesis by hydrothermal and Sol gel route method

27 March 2017 6,497 3 View

What is the meaning of Hill coefficient?

If we have a Hill coefficient equal to 2 or 2.5 or 2.7, what does it mean biologically or what is the interpretation of these numbers in biology?

18 July 2016 3,148 13 View

Does anyone can share the experience of larval oral secretions collection (Specifically from diamond back moth larvae)?

I want to collect the larval secretions of Diamonback moth larvae

11 May 2016 4,053 2 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How can we theoretically calculate Elemental analysis(percentage) of atoms in covalent organic framework?

For example in one research article the Elemental analysis values of COF-TpPa-1 ( Triformylphloroglucinol (Tp) (63 mg, 0.3 mmol), Paraphenylenediamine (Pa-1) (48 mg, 0.45 mmol) are as follows...

24 February 2021 1,265 6 View

How to Control Whiteflies in coconut leaves?

24 February 2021 1,005 1 View

What reaction will occur when adding hexamine into ammonium nitrate in an acidic medium?

Hello, I am investigating properties of water in oil emulsion explosives. And adding hexamine (hexamethylenetetramine) into one of the phases that are used to make the emulsion should increase the...

21 February 2021 8,680 1 View

How to get/count refractive index in gasses?

20 February 2021 8,202 6 View

Any Homemade Organic Synthesis Practices?

Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...

18 February 2021 2,769 4 View

How to find the capacitive response frequency from the impedance plot?

I have done electrochemical impedance by using cyclic voltammetry for one organic material. from that, I plotted bode diagram. but actually, I am very confused about how to find the capacitive...

18 February 2021 3,023 3 View

How to predict the reaction pathway for electro organic synthesis?

Can anyone recommend some any software/models available that can predict substance reactivities and the reaction yield under a specific set of reaction conditions process and proposing pathways to...

17 February 2021 2,383 3 View

Can bio-decomposer be a successful alternative to reduce residue burning?

To maintain the proper crop plan most of the times there is not enough time to make the field ready for the next crop. In this particular context , can bio-decompser be a solution? What about...

16 February 2021 7,068 24 View