Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Sample modeling in ABAQUS software and its relationship with the real world?

About crack modeling in ABAQUS: Mode I (pure-tensile cracking) and Mode II (pure-shear cracking), these assumptions may be accruing in theoretical approach (ABAQUS) not practical in Lab....

19 February 2021 6,156 1 View

absorption= 1-mag(S(FP1:1,FP1:1)*S(FP1:1,FP1:1))-mag(S(FP2:1,FP1:1)*S(FP2:1,FP1:1)) is it correct for absorption ?

hi everyone I am simulating a unit cell structure in HFSS using Flouquet port having two modes for absorption I have used this formula:- absorption=...

17 February 2021 9,178 2 View

An article on the Iranian state's civil liability in the COVID-19 matter needs supervision; Is anyone interested in collaborating?

Hello. An article on the Iranian state's civil liability in the COVID-19 issue has been written in collaboration with a friend and a comparative look at law and biology. The article is ready to be...

15 February 2021 2,906 2 View

Inter-rater / inter-coder reliability for thematic analysis?

In my research, I have used metaphorical elicitation tasks to investigate learners' conceptualization of speaking fluency. In the analysis phase, I used thematic analysis to make themes out of...

10 February 2021 4,719 11 View

How to define two different phases with different species?

Hello all, I am trying to model CO2 being dissolved in a bubble column filled with water. For mass transfer definitions, I need to define two phases: for instance, liquid and gas, where the liquid...

08 February 2021 2,722 3 View

What is the type or grade of ethanol used in SOL-GEL?

I'm preparing materials that will be use in SOL-GEL to produce silica gel, I will use Tetraethyl orthosilicate (TEOS) as precursor, and ethanol as solvent , but when I search to buy ethanol, the...

01 February 2021 5,153 4 View

Where to buy optically clear, low viscosity, refractive index 1.5-1.4 Silicone Oil ?

For research purposes, it is required to have optically clear, low viscosity silicone oil with a refractive index of 1.5-1.4. It is going to be used in optoelectronic research. Online purchase is...

01 February 2021 9,160 3 View

Can any one provide information about medicine, epidemic and critical issues or it is mandatory to be an expert ?

Due to the pandemic of coronavirus, the most source that the people depend on was social media. There is no guaranteed solution that is biased from political opinion. An example of that the...

01 February 2021 7,567 3 View

How can solve/disperse silicon nanoparticles in water?

I want to add silicon nanoparticles to my hydrogel, but when I sonicated these particles with water to disperse them in water, they agglomerated on the water's surface. How can I disperse them in...

26 January 2021 213 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View