The cell lines that I use are N2a and SH-SY5Y. What is the difference between inactivated or non-inactivated FBS?
Thanks in advance for your responses
the difference is complement proteins status. Complement proteins of FBS must be inactivated in 56c for 45min to preventing cell destruction.
Dear David,
Based on your experiment you can whether deactivate it or not.for instance, for experiments that include immune molecules it's necessary to deactivate FBS in order to prevent any intervention. But for any other type of test which immune molecules are not involved, it is not a must! You can look up the "FBS deactivation" in ATCC guide for animal cell culture.
Good luck
This strongly depends on your experimental setting. It is not necessary for simple cell culture, but needed to avoid stimulation from FBS possibly affecting specific experiments (e.g. stimulation with growth factors etc.).
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Cell Biology
- Culture Cells
- Cell Line