Is it necessary to confirm the data that obtained in 2-DE by other methods such as Western Blotting?

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Yannis Karamanos

To my oppinion the validation, of differences that have been obtained by a proteomics approach, is madatory. Western Blotting is one of the ways.

Miriam Khider

I think yes specially in diagnosis and medical purposes.

Masood Sepehrimanesh

Thanks dear Miriam

Thanks dear Yannis

Please discuse about other methods except western blot for confirmation of 2DE results