Is it necessary to confirm the data that obtained in 2-DE by other methods such as Western Blotting?

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Yannis Karamanos

To my oppinion the validation, of differences that have been obtained by a proteomics approach, is madatory. Western Blotting is one of the ways.

Masood Sepehrimanesh

Thanks dear Miriam

Thanks dear Yannis

Please discuse about other methods except western blot for confirmation of 2DE results