I need perform a T cell culture, but I don't know if is necessary to remove the endotoxins from antigen samples prior to use. Is there any problem if it don't remove the endotoxins to the samples?.
You only need to remove endotoxins if you had contamination in one of your reagents. Endotoxins only appear when there was bacteria/mold contamination added to your cultures/reagents at some point during processing. So are sure it's a problem? If you are using clean cells and cell applicable reagents, they all have certifications that show there is no endotoxins. However, if you have contamination somewhere during your processing there are a number of ways to remove it. Q Sepharose will remove it, or Lonza and cape cod associates both sell endotoxins test kits and removal resins. You can buy them through fisher/vwr. The best way to avoid endotoxins is to make sure you don't use contaminated reagents.
It would be desirable to remove the endotoxins in order to avoid possible cell stimulation by TLRs. While I agree that all reagents are majority endotoxin-free or present low levels of endotoxin (yes, you may find a low concentration of endotoxin in fetal calf serum), the antigen (recombinant produced by E. coli or crude antigens, in case of some bacterial contamination) may be an important source. You should check it by test using LAL kit. Alternatively, you could use polymixin B in the culture since it usually block the effect of the LPS...
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