Is it good to stop negative pressure to increase sealing resistance during patch clamp?

11 April 2023 1 3K Report

When I gave the negative pressure to seal the membrane, I found that if I released negative pressure during sealing, the sealing resistance increases quickly to reach above 1 gigaohms. Is that good or bad?

Xiaobo Wu

I did patch clamping on isolated cardiomyocytes.

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Science method

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