Does anyone know the pH-differential method?

I use the water method to extract the anthocyanidin from Peristrophe bivalvis (L.) Merr leave, then dilute 20 times with 0.025M potassium chloride, pH1.0 and 0.4M sodium acetate buffer, pH4.5...

20 January 2021 8,305 4 View

Has anyone tried cross-linking cells with formaldehyde before co-immunoprecipitation?

Hi, I am currently trying to co-immunoprecipitate a protein but the interaction between that protein and its binding partner is not stable. Sometimes we able to detect its binding partner but...

29 November 2020 9,261 3 View

TOP/FOP-FLASH assay in HEK293T cells?

Has anyone tried to study Wnt signaling in HEK293T cells? I have been trying to measure the Wnt activity using TOP/FOP-FLASH assay but the readings that I got are rather similar in both TOP and...

28 August 2020 6,052 4 View

Protocol for PC-3 spheroid (hanging drop) fixation and staining/IF?

Hi everyone, I am currently using the hanging drop method to generate spheroids of PC-3 prostate cancer cells. I would like to know if anyone has a detailed protocol I can use to fix these...

19 February 2020 2,756 1 View

Where to get a journal to publish highly technical marketing research based on multivariate analysis or old-fashioned statistical methods?

where to get a journal to publish highly technical marketing research based on multivariate analysis or old-fashioned statistical methods?

30 May 2019 2,711 2 View

How to determine the composition of a complex sample containing organic compounds, amorphous and crystalline compound containing copper?

The sample is an intermediate product in the synthesis of CuO-doped TiO2 catalyst. Ethanoic acid is added and it reacts with copper nitrate to produce copper(II)acetate. A gel is prepared with sol...

15 January 2019 6,796 7 View

Which surface cleaning procedures are recommended for steel fibers before coating?

in a catalytic coating experiment, steel fibers (stainless steel) will be coated with catalytic active CuO either through a) Sol gel methode or b) wet impregnation onto an intermediate coating...

04 November 2018 5,304 3 View

What is the green intermediate product of thermal decomposition of hydrated copper nitrate (copper(II) nitrate hemipentahydrate)?

When performing a synthesis of CuO-doped TiO2 nanoparticles through sol gel process (alkoxide path; ethanol as solvent), a bluish green sol and a green intermediate product (dried gel) were...

28 October 2018 5,051 3 View

How to bind zeolith particles onto the surface of ceramic fibers?

22 October 2018 1,516 2 View

Is it necessary to crosslink the RNA and protein while doing RNA binding protein immunoprecipitation?

06 September 2017 9,576 6 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View

Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS?

I need to model masonry and reinforced concrete minarets for my MSc thesis. Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS? (Or any other program for just...

01 March 2021 736 3 View

How to assign different material properties to each element in Abaqus ?

Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...

01 March 2021 9,511 3 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

How to modify Johnson cook equation in ABAQUS?

I wanted to add an extra parameter to the existing Johnson cook equation in the ABAQUS simulation. How to add those extra parameters. Ref: A modified Johnson-Cook material model with strain...

28 February 2021 4,841 3 View

How to improve and implement WBAN MAC and Radio modules under Castalia 3.2 ?

Hello researchers, Hello Castalia3.2 researchers I am a beginner with Castalia, I work on optimization of RFID systems used in wireless body networks. I simulated a WBAN with 10 nodes and one...

28 February 2021 400 1 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View