I have an O/N stock that I don't want to grow to stationary but am not able to make it in time to make the glycerol stock. Is it advisable to take it out of the shaking incubator and store it at 4 degrees centigrade until I am able to prepare the glycerol stock? Additionally, what OD is ideal for having the most effective glycerol stock that will last the longest?
Hi Veronica,
*It will depend on what culture you're working with*
A short answer would be: cells in the log phase (~0.8 OD), we believe, are good to prepare glycerol stocks. On storing the cells-- you can store it at 4C for now but make another O/N culture before you prepare a glycerol stock. You don't want to end up with a bad glycerol stock!
Cheers,
If you just wish to store the strain then you can make a frozen stock after storing for some hours or a day at 4oC.
It is OK to store the culture for few hours at 4 C before freezing them in a glycerol containing freezing medium.
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