How can writing tasks be designed or redesigned to accommodate the diversity in a multi -level ESL classroom?

Designing writing tasks in level- playing conditions of diverse groups in ESL settings calls for structural adjustment with the established notions of task design features. Yet , the culture of...

21 October 2020 9,469 6 View

Is the heat treatment in open atmosphere an effective method for decrease carbon on surface graphitic cast iron?

The target is removing or decreasing free carbon on the external surface of gray and ductile iron.

09 August 2020 612 2 View

How to Convert large fastq file to fasta?

Hello, I'm new to bioinformatics, my NGS dataset consists of 50 FASTQ files and each file is of size 10-15 GB.. I need to convert these FASTQ files to FASTA files. P.S. I am using google colab I...

02 July 2020 6,224 2 View

I want an open source FDTD for SAR signal correction due to propagation in ionosphere?

SAR signal processing software or matlab code for SAR signal processing specially for SAR signal corrections due to propagation in ionosphere

26 March 2020 5,855 3 View

Why Researchers Dissolve Sodium Azide in Phosphate Buffer (0.1M) in mutagenasis?

Really i have three questions, if i can get answers about it! 1- First One, Usually researchers used Sodium Azide with concentrations (1-5 mM or 0.01-0.1%) with long time (15-20 hour) on pH=7, or...

07 October 2019 9,045 2 View

How can I store stool specimen for Molecular diagnosis for human parasites Is there a difference of storing for PCR or real PCR or multiplex ?

Molecular stool

08 January 2019 8,186 3 View

RNA Quantity & Quality extracted from Escherichia coli in M9 supplemented with different sugars?

I am trying to extract RNA from Escherichia coli grown in M9 minimal media supplemented with different sugars, however, every time the RNA extract ranges from 25 to 50 ng/ul. I am using the Qiagen...

23 October 2018 992 2 View

Is there an online source to know the equivalence of each OD to cfu/ml for Escherichia coli?

Is it the same for all media or differs from LB to minimal media? Doesn't OD correlate to cell mass? To be sure should i do a viable count for the growth of E.coli in specific media?

20 October 2018 2,588 3 View

Best Gel documentation system ?

02 April 2018 6,383 4 View

Electroporation of cripr cas9 by gene pulser?

Kindly I need to do transfection for canecr cell lines by Crispr cas 9 from santa cruiz (downregulation and overexpression) , I wonder if cuvette 0.2 will work with it as I don't have 0.4 and the...

28 March 2018 3,398 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

How can I retrieve FASTA sequence from gene ID or UID of a Gene ? I have GeneBank IDs ?

I treid to use Entrez Batch, but it redirects me to NCBI site. Is there any script of Linux or Python through which I can download Fasta sequence of list of gene ID ?

21 February 2021 7,783 12 View

Which CpGs have a significant effect on gene expression?

It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...

20 February 2021 5,634 3 View

Blast analysis of RC,UHPC slabs using material model *MAT_72R3 in LS-DYNA?

20 February 2021 5,984 3 View