Is the heat treatment in open atmosphere an effective method for decrease carbon on surface graphitic cast iron?

The target is removing or decreasing free carbon on the external surface of gray and ductile iron.

09 August 2020 612 2 View

I want an open source FDTD for SAR signal correction due to propagation in ionosphere?

SAR signal processing software or matlab code for SAR signal processing specially for SAR signal corrections due to propagation in ionosphere

26 March 2020 5,855 3 View

Why Researchers Dissolve Sodium Azide in Phosphate Buffer (0.1M) in mutagenasis?

Really i have three questions, if i can get answers about it! 1- First One, Usually researchers used Sodium Azide with concentrations (1-5 mM or 0.01-0.1%) with long time (15-20 hour) on pH=7, or...

07 October 2019 9,045 2 View

How to calculate MDA content from absorbance values at 532 and 600 nm?

in analysing MDA content in plant tissues, I took 0.2 g leaf tissue (fresh welght), added 5 ml 0.1% TCA and samples were grind. samples then were centrifuged at 12500 rpm for 20 minutes. 3 ml from...

16 July 2019 6,884 2 View

Does BHK (Baby Hamster kidney) cell line have specific regulations to Enter Egypt?

I need to import BHK cells to Egypt, but I'm not sure if there are any regulations for it to Enter!

26 February 2019 4,814 1 View

How you calculate the sample size for project on a clinical disease?

How you calculate the sample size for project on a clinical disease?

21 July 2018 9,389 9 View

No title

how prepare this mix morality in constant total volume 800 ul

04 June 2018 9,451 3 View

Can somebody tell me the most common microorganism isolated from ICU?

Can somebody tell me the most common microorganism isolated from ICU?

23 July 2017 9,542 5 View

Why some planes in orthorhombic structure have four miller indices?

we know the orthorhombic structure has three axes and hexagonal structure has four axes. therefore, the miller indices for orthorhombic structure and hexagonal structure are (hki) ,(hkil)...

13 February 2017 5,956 3 View

No title

07 January 2017 7,604 2 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View