Nehal Mohammed Ramadan

5 Questions 15 Answers 0 Followers

Questions related from Nehal Mohammed Ramadan

What is the best product size of SYBR Green Real-Time PCR to amplify c-DNA?

I have designed a primer, the expected product length is 51, according to NCBI, is this product length accepted for real-time PCR?

05 August 2016 2,175 9 View

Is it acceptable for the primer in real time PCR to be specific for a variant of the gene along with the target gene??

I am designing a primer for real time PCR and when I blasted the primer on NCBI, it appear to be specific to the target gene, but also appear to have specificty on a transcript variant of this...

04 August 2016 6,292 5 View

Why I get a blank signal on western blot?

Hi, I am new to western blotting and so facing the problem of blank blots. i want to assess the expression of two membrane bound proteins, 45 & 74 KD, in rat heart tissue. My tissue samples...

08 September 2015 9,951 13 View

How long should I store my antibody solution after first opening of the vial?

I have an antibody solution at a concentration of 200 micrograms/ml, as supplied by the manufacturer. I stored it at 4 degree, as stated in the data sheet. I will use this 200 micrograms for...

10 January 2015 2,540 9 View

What is the difference between polyclonal and monoclonal antibodies?

As regard western blot, which of the two types of antibodies will detect the total amount of protein, either phosphorylated or not?

01 April 2014 8,202 7 View